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Specimen Collection
 

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Parasitology Specimen Collection Guidelines

 

 

Specimen

 

Type

 

Collectiona

 

 

 

Transport

 

Time and

 

Temp to lab

 

 

 

Guidelines

 

Device

 

Preservative

 

Minimum Vol

 

Replica limits

 

Comments

 

   Unpreserved

 

Parasite and cyclical peak:

 

Ascaris lumbricoides,

 

constant

 

Dientamoeba fragilis, irregular

 

Diphyllobothrium latum, irregular

 

E. histolytica, 7-10 days

 

Giardia lamblia, 3-7 days

 

Hookworm, constant

 

Trichuris trichiura constant;

 

Schistosoma spp., irregular

 

Sterile, leakproof, wide-mouth container

 

Nonea

 

5g

 

1/day

 

Liquid: < 30 min, RT

 

Semisolid: < 1 h, RT

 

Formed: < 24 h, 4oC

 

 

 

Pinworm paddle

 

1. Gently press the paddle's stick side against several areas of the perianal region which spreading open the perianal folds.

 

2. Place the paddle in the transport container and tighten the cap.

 

3.  Daily consecutive specimens (>6) should be obtained before patient is consideGold infection-free.

 

Pinworm paddle kit

 

None

 

None

 

1/day

 

< 24 h, RT

 

Common parasites:

 

Enterobius vermicularis, Taenia spp.

 

Specimens are best obtained at 10-11 p.m. or upon waking and before a bowel movement or both.

 

Wash hands after collection.

 

Skin snip

 

1.  A sharp razor blade can be used to obtain a sample of skin which may be so superficial that no bleeding occurs.

 

2.  Alternatively, a needle can be used to raise the skin and with a scalpel remove the skin below the needle.

 

3.  Any body location is satisfactory but the middorsal region just to one side of the midline is frequently selected.

 

4. Place the skin snip in a tube containing 0.2-0.4 ml. of saline.

 

Sterile tube

 

Sterile saline

 

None

 

None

 

< 30 min, RT

 

Common parasites:

 

Mansonella

 

streptocerca,

 

Onchocerca volvulus

 

Skin ulcer

 

1. Obtain scrapings or biopsies of the active margin of cutaneous or mucocutaneous ulcers.  A punch biopsy is recommended.

 

2. Place sample in a sterile tube containing enough sterile saline to keep it moist.

 

Sterile tube

 

Sterile saline

 

None

 

1/day/site

 

< 30 min, RT

 

Common parasites:

 

Acanthamoeba spp.; Entamoeba histolytica;

 

Leishmania spp.

 

 

 

 

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